ASTM E1873-2006 用聚合酶链式反应技术测定核酸序列的标准指南
时间:2024-05-19 23:38:07 来源: 标准资料网 作者：标准资料网 阅读：8970
【英文标准名称】:StandardGuideforDetectionofNucleicAcidSequencesbythePolymeraseChainReactionTechnique
【原文标准名称】:用聚合酶链式反应技术测定核酸序列的标准指南
【标准号】:ASTME1873-2006
【标准状态】:现行
【国别】:
【发布日期】:2006
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:生物工艺学;链式;检测;微生物学;核酸;序列
【英文主题词】:amplification;diagnosis;DNAprobe;hybridization;PCR;polymerasechainreaction;primer
【摘要】:ThisguideisintendedforuseinanylaboratoryutilizingPCRorRT-PCRtoamplifyanddetectaspecificnucleicacidsequence.ThecriteriausedforevaluationoftheamplificationreactionsshouldbeadministeredbyanindividualtrainedintheuseofmolecularbiologicaltechniquesassociatedwithPCR.1.1Thisguidecoversguidelines,recommendations,basicconsiderations,criteria,andprinciplestobeemployedwhendeveloping,utilizing,orassessingPCRproceduresandspecificprotocolsfortheamplificationanddetectionofnucleicacidsequences.ThisguideisnotintendedtobeastandardprocedurewithalistofrequirementsforPCRdetectionofnucleicacids.Thisguideisintendedtoprovideinformationthatwillassisttheuserinobtainingqualityandreliabledata.1.2NucleicacidtargetsforPCRincludeDNA,aswellasRNA;RNAsequencesaresuitabletargetsforPCRfollowingreversetranscriptionoftheRNAtocomplementaryDNA(cDNA).Thistypeofamplificationtechniqueisknownasreversetranscription-PCR(RT-PCR).1.3Thisguidehasbeendevelopedforuseinanymolecularbiology/biotechnologylaboratory.Thisincludes,butisnotlimitedto,laboratoriesthatspecializeinthediagnosisofhuman,animal,plant,orbacterialdiseases.1.4ThisguideconveysthegeneralproceduralterminologyofPCRtechnologyusedforthedetectionofnucleicacids.1.5Thisguideisgeneral;itdoesnotcovertheadditionalguidancethatwouldbeneededforspecificapplications,forexample,forthePCRdetectionofnucleicacidsequencesofspecificmicroorganisms.1.6ThisguidedoesnotcoverdetailsofthevariousmethodsthatcanbeutilizedtoidentifyPCR-amplifiedDNAsequences.1.7ThisguidedoesnotcoverspecificvariationsofthebasicPCRorRT-PCRtechnology(forexample,quantitativePCR,real-timePCR,multiplexPCR,andinsituPCR),anditdoesnotcoverdetailsofinstrumentcalibration.1.8Warning-Laboratoryworkinvolvingcertainclinicalspecimensandmicroorganismscanbehazardoustopersonnel.Warning-Biosafetylevel2(orhigher)facilitiesarerecommendedforbiohazardwork().SafetyguidelinesshouldbeadheredtoinaccordancewithCLSIM29-A2andotherrecommendations().
【中国标准分类号】:A40
【国际标准分类号】:07_100_99
【页数】:9P.;A4
【正文语种】:
【英文标准名称】:Thermalspraying-Pre-treatmentofsurfacesofmetallicpartsandcomponentsforthermalspraying;GermanversionEN13507:2010
【原文标准名称】:热喷涂.热喷涂金属部件和元件表面预处理.德文版本EN13507-2010
【标准号】:DINEN13507-2010
【标准状态】:现行
【国别】:德国
【发布日期】:2010-06
【实施或试行日期】:
【发布单位】:德国标准化学会(DE-DIN)
【起草单位】:
【标准类型】:()
【标准水平】:()
【中文主题词】:元部件;材料;金属喷镀;金属的;金属表面;金属;准备;工艺规范;规范(验收);喷雾;喷射;表面处理;表面;热喷涂;焊接工程;工件
【英文主题词】:Components;Materials;Metalspraying;Metallic;Metallicsurfaces;Metals;Preparation;Processspecification;Specification(approval);Spray;Spraying;Surfacetreatment;Surfaces;Thermalspraying;Weldingengineering;Workpieces
【摘要】:
【中国标准分类号】:A29
【国际标准分类号】:25_220_10
【页数】:12P;A4
【正文语种】:德语
【英文标准名称】:StandardTestMethodsofCompressionTestingofMetallicMaterialsatRoomTemperature
【原文标准名称】:室温下金属材料压缩试验的标准试验方法
【标准号】:ASTME9-2009
【标准状态】:现行
【国别】:美国
【发布日期】:2009
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E28.04
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:
【摘要】:Significance8212;Thedataobtainedfromacompressiontestmayincludetheyieldstrength,theyieldpoint,Young''smodulus,thestress-straincurve,andthecompressivestrength(seeTerminologyE6).Inthecaseofamaterialthatdoesnotfailincompressionbyashatteringfracture,compressivestrengthisavaluethatisdependentontotalstrainandspecimengeometry.Use8212;Compressivepropertiesareofinterestintheanalysesofstructuressubjecttocompressiveorbendingloadsorbothandintheanalysesofmetalworkingandfabricationprocessesthatinvolvelargecompressivedeformationsuchasforgingandrolling.Forbrittleornonductilemetalsthatfractureintensionatstressesbelowtheyieldstrength,compressiontestsofferthepossibilityofextendingthestrainrangeofthestress-straindata.Whilethecompressiontestisnotcomplicatedbyneckingasisthetensiontestforcertainmetallicmaterials,bucklingandbarreling(seeSection3)cancomplicateresultsandshouldbeminimized.1.1Thesetestmethodscovertheapparatus,specimens,andprocedureforaxial-loadcompressiontestingofmetallicmaterialsatroomtemperature(Note1).Foradditionalrequirementspertainingtocementedcarbides,seeAnnexA1.Note18212;Forcompressiontestsatelevatedtemperatures,seePracticeE209.1.2Thevaluesstatedininch-poundunitsaretoberegardedasstandard.ThevaluesgiveninparenthesesaremathematicalconversionstoSIunitsthatareprovidedforinformationonlyandarenotconsideredstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:H22
【国际标准分类号】:77_040_10
【页数】:10P.;A4
【正文语种】:英语